Rodolfo Paoletti; David Kritchevsky's Advances in lipid research. Vol. 18, 1981 PDF

By Rodolfo Paoletti; David Kritchevsky

ISBN-10: 0120249189

ISBN-13: 9780120249183

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1973). However, to obtain the large quantities needed for biochemical studies, the " d e r ­ m a t o m e " method developed by Roberts et al. (1974) is the m e t h o d of choice. It involves using an electrical " d e r m a t o m e " as in plastic surgery, with adjustment of the cutting depth from a minimum of 10 pan. , 1974). g. Hautchen Preparations. , peeled off pieces of endothelium m o u n t e d flat for microscopic examination. Some methods described for making H a u t c h e n preparations involved fixation and alcoholic dehydration of the tissue.

After this, one progresses to electron microscopy to observe the relation­ ship and morphology of the subcellular structure. Due to this relatively new optical instrument and its modifications, information regarding cells and subcellular organelles has been accumulated. Thus microsomes or ribosomes of the cytoplasm, concerned with reproduction and protein syn­ thesis, mitochondria with enzyme-metabolic activities, and the lysosomes, have been elucidated by these instruments. The electron microscope thus serves as a bridge between the light microscope and the interatomic dimen­ sions studied by X rays.

The reader is also referred to R h o d i n ' s " H i s t o l o g y " (1973) for more details on electron microscopy of normal cardiovascular systems. Constantinides (1974) points t o an intimate relationship between ultrastruc­ ture and cell function, suggesting that "structure is really frozen function and function is nothing but structure in m o t i o n . " The introduction of electron microscopy in this field has added meaning to the results already obtained by biochemical methods as to the composi­ tion and function of cellular organelles.

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Advances in lipid research. Vol. 18, 1981 by Rodolfo Paoletti; David Kritchevsky


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